Fluorescent europium-modified polymer nanoparticles for rapid and sensitive anthrax sensors |
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Authors: | Oh Wan-Kyu Jeong Yoon Seon Song Jooyoung Jang Jyongsik |
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Institution: | World Class University (WCU) Program of Chemical Convergence for Energy & Environment (C(2)E(2)), School of Chemical and Biological Engineering, Seoul National University, 599 Gwanangro, Gwanak-gu, Seoul 151-742, Republic of Korea. |
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Abstract: | Novel fluorescent polyacrylonitrile nanoparticles were synthesized by microemulsion polymerization and Schiff base modification. By further modification with europium, the polyacrylonitrile nanoparticles could be used as a highly sensitive and rapid sensor for Bacillus anthracis spore detection in aqueous solution. The europium-modified polyacrylonitrile nanoparticles were readily combined with dipicolinic acid as a unique biomarker of B. anthracis, leading to high fluorescence emission. These nanoparticles enabled ratiometric detection without instrument-specific calibration due to the internal fluorescence reference. Additionally, the europium-modified polyacrylonitrile nanoparticle sensors exhibited a remarkable limit of detection (10pM) for dipicolinic acid and outstanding selectivity (160×) over aromatic ligands in aqueous solution. The ultrafine nanoparticle sensor showed a high capability for detecting anthrax due to the increased surface area-to-volume ratio and enhanced dispersibility. |
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