Abundance and half-life of the distinct oat phytochrome A3 and A4 mRNAs |
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Authors: | David C Higgs Linda J Barnes James T Colbert |
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Institution: | (1) Department of Botany, Iowa State University, 50011 Ames, IA, USA;(2) the Molecular, Cellular, and Developmental Biology Program, Iowa State University, 50011 Ames, IA, USA;(3) Present address: Boyce Thompson Institute for Plant Research, Cornell University, 14853 Ithaca, NY, USA |
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Abstract: | Gene-preferential oligonucleotide probes were used to determined the relative abundance and half-lives of distinct oat phytochrome A (PHYA) mRNAs. Oat PHYA mRNAs are highly conserved in the 5 -untranslated region and the coding region, but the 3 -untranslated region has an overall lower sequence conservation and was the source of gene-preferential probes. PHYA3 mRNA was estimated to be ca. 61% of the oat PHYA mRNA pool present in poly(A)+ RNA from dark-grown seedlings. The half-lives for PHYA3 and PHYA4 mRNAs were both estimated to be ca. 30 min, and a similar short half-life was estimated for the average PHYA mRNA. Sequence comparisons of PHYA mRNAs from four grass species identified conserved sequences within the 5 - and 3 -untranslated regions that might be important for PHYA mRNA degradation. |
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Keywords: | Avena sativa gene expression PHYA light regulation mRNA degradation |
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