A sensitive colorimetric assay for mitochondrial alpha-glycerophosphate dehydrogenase |
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Authors: | R S Gardner |
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Affiliation: | Department of Internal Medicine, University of Iowa, and Veterans Administration Hospital, Iowa City, Iowa 52242 USA |
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Abstract: | A simple reproducible assay for mitochondrial α-glycerophosphate dehydrogenase (GPD) has been described. It takes advantage of the ability of 2-p-iodo-3-p-nitro-5-phenyl tetrazolium chloride (INT) to directly accept electrons from the dehydrogenase. The assay will accurately measure the enzyme using 20 μg of mitochondrial protein, but it could be scaled down further using smaller volumes and microcuvettes. |
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