Defective sterol Δ<sup><nobr>5(6)</nobr></sup>desaturase as a cause of azole resistance in Ustilago maydis期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Defective sterol Δ⁵⁽⁶⁾desaturase as a cause of azole resistance in Ustilago maydis

Authors:	Timothy Joseph-home Nigel J Manning Derek Hollomon Steven L Kelly

Institution:	Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2UH, UK; Neonatal Screening Laboratory, Sheffield Children Hospital, Western Bank S10 2UH, UK; Department of Agricultural Sciences, University of Bristol, Institute of Arable Crops Research, Long Ashton Research Station, Long Ashton, Bristol, UK

Abstract:	Abstract Resistance to azole antifungals in Ustilago maydis was associated with a leaky defect in sterol Δ ⁵⁽⁶⁾desaturase. This defect resulted in reduced accumulation of 14α-methylergosta-24(28)-diene-3β, 6 α-diol and an increase in the proportion of 14α-methylfecosterol in treated cells when compared to the parent strain. The results demonstrate the importance of this mechanism in pathogenic fungi.

Keywords:	Azole Resistance P450 Sterol desaturase Ustilago maydis