Binding and penetration ofRhizobium japonicum to cultured soybean cells |
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Authors: | Siu-Cheong Ho Shahnaz Malek-Hedayat |
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Affiliation: | (1) Department of Biochemistry, Michigan State University, 48824 East Lansing, MI, USA |
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Abstract: | A cultured soybean cell line, SB-1 was used to evaluate the initial interaction between the soybean cells andRhizobium japonicum. Co-culturing ofR. japonicum with SB-1 cells in suspension resulted in strain-specific polar attachment. This attachment can be inhibited by galactose and antibodies raised against seed soybean agglutinin (SBA). A lectin was purified from SB-1 cells which shares properties with SBA in terms of immunological reactivity, sugar binding activity, polypeptide molecular weight and peptide maps. When the SB-1 cells were co-cultured withR. japonicum for three weeks in solid agar medium, histological staining revealed bacterial penetration into certain SB-1 cells. Furthermore, there were focal regions of cells with prominent nuclei representing actively proliferating regions. These observations are analogous to that ofin vivo nodule initiation in soybean roots. |
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Keywords: | soybean agglutinin SB-1 lectin homology bacterial binding lectin hypothesis host specificity |
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