Effects of taurine on the phosphorylation of specific proteins in subcellular fractions of the rat retina |
| |
Authors: | John B Lombardini |
| |
Institution: | (1) Departments of Pharmacology and Ophthalmology and Visual Sciences, Texas Tech University Health Sciences Center, 79430 Lubbock, TX |
| |
Abstract: | The effects of 20 mM taurine on the phosphorylation of specific proteins in mitochondrial and rod outer segment subcellular fractions of the rat retina were measured. A band of protein with an apparent molecular wieght of 20K was consistently inhibited by taurine. Densitometry measurements performed on gel electrophoresis autoradiograms from the mitochondrial fraction demonstrated a 42.7±8.3% decrease due to taurine (20 mM) in the area corresponding to radioactivity from the 20K phosphoprotein. However, only a 21.2±9.0% decrease was observed due to taurine in the rod outer segment preparation. These data suggest that taurine is exerting its primary effect on the phosphorylation of the 20K molecular weight protein in the mitochondria of the retina. In addition, calmodulin and phorbol ester had no effect on the phosphorylation of the 20K molecular weight protein. |
| |
Keywords: | Taurine retina mitochondria rod outer segments phosphorylation |
本文献已被 SpringerLink 等数据库收录! |