华南地区新城疫病毒HN基因的克隆及其系统发育分析

用RT PCR一步法扩增了华南地区NDV野毒HN基因 ,并对其中的 3株的HN基因 897bp片段进行了克隆、测序及同源性分析。本试验还对该毒株的系统发育情况进行了分析 :中国华南地区NDV各毒株与欧美国家Ⅰ至Ⅴ基因型明显分离 ,遗传关系较远。台湾省的NDV各毒株归属为一型 ,并且与华南株的遗传距离较近。NDV华南FS1株不能归类到任何一个基因型中。广东地区从鹅群中分离到的副粘病毒GPMV株与我们从鸡体中获得的NDVFS2株有很近的亲缘关系

Study on the HN Gene Cloning of NDV Isolates in South China and their Phylogenesis

Three HN genes of NDV isolates from South China were amplified by one-step RT-PCR. They where subsequently cloned and sequenced. The Phylogenesis of these NDV isolates was also studied. The gene types of South China NDV strains were found to be separated from those of Euro-American NDV strains exhibiting farther genetic distance. NDV isolates from Taiwan Province belonged to the same gene type as those from Mainland China. However,one of the Chinese NDV strain, FS1, belonged to none of these gene types. Significantly, a paramyxovirus strain, named GPMV that was isolated from Guangdong Province, had a rather close genetic relationship with FS2 stain that was isolated from chicken in Guangdong.