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Adaptation of a Silica Membrane-based Kit for Purification of Sequencing-ready BAC DNA |
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| Authors: | C. Eduardo Vallejos Ney S. Sakiyama Valerie Jones Savita Shanker |
| Affiliation: | 1. Department of Horticultural Sciences and Plant Molecular and Cellular Biology Program, University of Florida, 1143 Fifield Hall, Gainesville, FL, 32611-0690, USA 2. Departamento de Fitotecnia, Universidade Federal de Vi?osa, Vi?osa, MG, Brazil 3. DNA Sequencing Laboratory, Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, FL, 32611, USA |
| Abstract: | We describe a modification of a protocol for the isolation of BAC DNA using a silica membrane-based kit designed for the isolation of plasmid DNA. The major advantages of this protocol are the expediency of the procedure, the high yield and purity, and the high quality of the BAC DNA that is suitable for direct sequencing. |
| Keywords: | BAC DNA purification Direct BAC DNA sequencing BAC fingerprinting |
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