Measurement of DNA length by gel electrophoresis. I. Improved accuracy of mobility measurements using a digital microdensitometer and computer processing |
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| Authors: | J K Elder A Amos E M Southern G A Shippey |
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| Affiliation: | 1. MRC Clinical and Population Cytogenetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU United Kingdom;2. MRC Mammalian Genome Unit, Department of Zoology, University of Edinburgh, King''s Buildings, West Mains Road, Edinburgh EH9 3JT, United Kingdom |
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| Abstract: | The electrophoretic mobilities of DNA polymer fragments in an agarose gel have been measured from a photograph of the gel by different methods and converted to lengths by the reciprocal method. The method of measurement can introduce large errors in the length estimates. The use of a digital microdensitometer to obtain optical density profiles of gel tracks with subsequent computer processing to find peak positions was found to give the most accurate DNA lengths. |
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| Keywords: | To whom all correspondence should be addressed. |
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