Arabidopsis ent-Kaurene Oxidase Catalyzes Three Steps
of Gibberellin Biosynthesis |
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| Authors: | Chris A Helliwell Andrew Poole W James Peacock and Elizabeth&#; S Dennis |
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| Institution: | Chris A. Helliwell, Andrew Poole, W. James Peacock, and Elizabeth
S. Dennis |
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| Abstract: | The Arabidopsis GA3 cDNA was expressed in yeast (Saccharomyces cerevisiae) and the ability of the transformed yeast cells to metabolize ent-kaurene was tested. We show by full-scan gas chromatography-mass spectrometry that the transformed cells produce ent-kaurenoic acid, and demonstrate that the single enzyme GA3 (ent-kaurene oxidase) catalyzes the three steps of gibberellin biosynthesis from ent-kaurene to ent-kaurenoic acid. |
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