Isolation and properties of malate dehydrogenase from Meso-and thermophilic bacteria |
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Authors: | A T Eprintsev M I Falaleeva M A Klimova N V Parfenova |
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Institution: | (1) Voronezh State University, Voronezh, 394006, Russia |
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Abstract: | A scheme of purification of malate dehydrogenase from Macromonas bipunctata strain D-405 and Vulcanithermus medioatlanticus DSM 14978T was developed. This scheme was used to obtain electrophoretically homogeneous enzyme preparations of the mesophilic bacterium M. bipunctata (specific activity, 26.9 ± 0.8 U/mg protein; yield, 10.9%) and the thermophilic bacterium V. medioatlanticus (specific activity, 5.0 ± 0.2 U/mg protein; yield, 19.2%). Using these high-purity enzymatic preparations, the physicochemical and regulatory properties of malate dehydrogenase were studied and the differences in kinetic characteristics and thermal stability of the preparations were determined. |
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