厚壳贻贝低分子量足丝蛋白的初步鉴定(英文)

足丝蛋白是贻贝科(Mytilidae)所特有一种在水环境中也能表现出强黏附功能的蛋白,也是目前开发新型生物黏附剂的主要候选分子。厚壳贻贝(Mytilus coruscus)广泛分布于我国东部沿海,是我国具有重要经济价值的贻贝,其足丝粗硬,黏附力强,关于厚壳贻贝的足丝蛋白的研究目前尚未见报道。通过醋酸抽提结合反相高效液相色谱分离,从厚壳贻贝足丝盘中分离纯化到数种低分子量足丝蛋白,经质谱鉴定和氨基酸序列测定,其中三种足丝蛋白(分子量6 kD左右)属于贻贝足丝蛋白-3(mytilus foot protein-3,mfp-3)家族,且序列中富含DOPA,另有三种足丝蛋白为未知新型足丝蛋白。石英晶体微天平分析表明,厚壳贻贝低分子量足丝蛋白在金表面有较强的吸附能力,这与其黏附功能是直接相关的。以上工作为深入了解厚壳贻贝低分子量足丝蛋白的分子多样性以及黏附机制奠定了基础。

IDENTIFICATION OF MUSSEL FOOT PROTEINS WITH LOW MOLECULAR MASS FROM MYTILUS CORUSCUS PLAQUES

Mytilidae owns a kind of special adhesive protein known as the mytilus foot protein (mfp) which provides secure adhesion in the presence of moisture and therefore become the main candidate for new biological adhesive developing at present. Possessing important economic value, Mytilus coruscus, with strong byssul and excellent adhesion, widely spreads all through the littoral extent of Zhejiang, China, while its foot proteins had rarely been studied. To understand the composition and the characterization of adhesive proteins from Mytilus coruscus several foot proteins were separated by C18 reverse-phase HPLC from the acetic acid-urea extracts of plaques and analyzed by matrix-assisted laser desorption ionization time of flight mass spectrometry to determine the mass ranging from3 to 6 kD followed with partially sequencing by Edman degradation. Three of these proteins, containing abundant amount of Dopa, were deter-mined to be variants of a family known as mfp3 (mytilus foot protein 3) and the other three proteins were presumed to be novel types of foot proteins, because no results were matched after online BLAST search. Quartz crystal microbalance analysis assay showed that some of these proteins exhibited strong absorption abilities on gold surface, which directly correlated with their adhesion force. Notwithstanding its limitation, this study did build the foundation for further re-search on the molecular diversity of the foot proteins with low molecular mass from Mytilus coruscus and the related adhesion mechanism.