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Simultaneous monoamine histofluorescence and neuropeptide immunocytochemistry |
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| Authors: | Dr. John R. Sladek Jr. Thomas H. McNeill |
| Affiliation: | (1) Department of Anatomy, Center for Brain Research, University of Rochester, School of Medicine and Dentistry, Rochester, New York, USA;(2) Department of Neurology, University of Rochester, School of Medicine and Dentistry, Rochester, New York, USA;(3) Department of Anatomy, University of Rochester, School of Medicine, 601 Elmwood Avenue, Box 603, 14642 Rochester, NY, USA |
| Abstract: | Summary A method was developed that allows the analysis of neuropeptides and monoamines in a single tissue section by the application of the unlabeled antibody method for peptide staining to tissue sections freeze-dried for formaldehyde-induced monoamine histofluorescence. The hypothalamic magnocellular system of male albino rats served as a model for this study; neurons were stained with anti-neurophysin sera, which mark the vasopressin- and oxytocin-associated proteins. Neurophysin-containing perikarya appeared to be surrounded by catecholamine-containing varicosities. This phenomenon was seen to varying degrees within the supraoptic and paraventricular nuclei. The juxtaposition of varicosities and peptidergic neurons suggests an afferent fiber-target neuron relationship that might favor a functional interaction between monoamines and neuropeptides.Supported by USPHS Grant and postdoctoral NS15816 (JRS) fellowship AG01575 (THM) |
| Keywords: | Catecholamines Neurophysin Simultaneous demonstration Functional interaction Rat |
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