PARTIAL PURIFICATION OF FRUCTOSE 1,6-BISPHOSPHATASE FROM THE MARINE MACR0ALGA BRYOPSIS CORTICULANS (CHLOROPHYCEAE)1

Fructose 1,6-bisphosphntase (FRPase, EC 3.1.3.11) partially purified from the marine green seauleed Bryopsis corticulans Setch exhibited a single active Peak on a Sephadex G-200 column but two distinct peaks on a DEAE cellulose column. The two fractions shared the same molecular weight of 162,000 as revealed by gel filtration. The FBPase from Bryopsis was unstable in storage elen zlheii an appropriatr amount of 2-mercaptoethnnol was present unless Mn²⁺and glycerol uere added in the buffer. Preliminary results indicate that the FBPase is of chloroplastic origin.