Electron microscope study of ribonucleoprotein polyparticles and their relation to perichromatin granules |
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Authors: | Gerardo Vazquez-Nin Alma Ortiz Aurora Lopez Olga Echeverria |
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Abstract: | Nuclear ribonucleoprotein particles were studied in the central nervous system of the rat after fixation by perfusion with hypotonic-detergent formaldehyde. Beads-on-a-string structures (polyparticles or chains) formed of 14-nm granules related by a 7-nm thick filament were found. These polyparticles are positively contrasted by a preferential method for ribonucleoproteins and they are sensitive to RNase. Perichromatin granules are loosened by this fixation and their internal structure is clearly depicted. They are composed of a tangled mass of 3-nm thick filaments, but lack 14-nm granules. The internal filaments of the perichromatin granules display frequent continuities with polyparticles. These results show that polyparticles correspond to perichromatin fibrils visualized in nuclei by standard fixation procedures. |
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Keywords: | nucleus interphase ribonucleoproteins nuclear particles |
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