Interaction of seminalplasmin with chlortetracycline, a fluorescent chelate probe of Ca2+ |
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Authors: | S Shivaji |
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Affiliation: | Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India |
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Abstract: | The interaction of seminalplasmin with chlortetracycline, a fluorescent chelate probe of Ca2+, was studied. The results indicate that seminalplasmin binds to chlortetracycline. The binding is not influenced by salt. Both Ca2+ and seminalplasmin probably bind to the same site on chlortetracycline. Seminalplasmin also reduced the Tb3+-associated fluorescence of bovine spermatozoal plasma membrane. These results are discussed in relation to the inhibitory effect of seminalplasmin on the uptake of Ca2+ in bovine spermatozoa. |
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