Cerium-Based Demonstration of Phosphatas Activity in Plastic-Embedded Sections: A Comparison with Conventional Methods |
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| Authors: | Harry van Goor Klaas Poelstra Machiel J Hardoi |
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| Institution: |
a Department of Pathology, University of Groningen, Groningen, The Nethe |
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| Abstract: | Cerium-based methods have been used for the demonstration of seve phatases at alkaline, acid and neutral pH in low temperature acetone-fixed, plastic-e sections. At alkaline pH calcium is used as capturing agent and the precipitated phosphate converted to cerium phosphate. At neutral and acid pH cerium is used d capturing agent. Cerium phosphate is subsequently visualized using the H2C2DAB n: comparison has been made with conventional calcium-cobalt and lead methods. It; that calcium-cobalt methods are more susceptible to improvement than lead methods |
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