RNA synthesis in germinating red bean seeds |
| |
Authors: | Watanabe, Akira Nitta, Takeshi Shiroya, Tsugio |
| |
Affiliation: | Laboratory of Radiation Biology, Faculty of Science, University of Tokyo Hongo, Bunkyo-ku, Tokyo, Japan |
| |
Abstract: | Nucleic acids from 32P-labelled germinating red bean seeds wereinvestigated by means of MAK column chromatography. 1) In cotyledons,synthesis of D-RNA occurred in the early stages of germination,3 to 24 hr after the onset of imbibition. 32P was also incorporatedinto rRNA continuously at rather a moderate rate. DNA-RNA hybridizationexperiments revealed that the proportion of heterogeneous RNA(D-RNA) to rRNA decreased gradually. Nucleotide analysis suggestedthat tRNA was synthesized de novo, and that its CCA-end exchangewas remarkable at early stages of germination. 2) In embryos,however, the incorporation of 32P into rRNA was very much greaterthan into D-RNA, and the exchange reaction at CCA-end of tRNAwas not detected. The role of D-RNA, found in cotyledons inthe initial stages of germination, was discussed. 1Present address: Research Institute for Biochemical Regulation,Faculty of Agriculture, Nagoya University, Chikusa-ku, Nagoya,Japan. (Received May 10, 1972; ) |
| |
Keywords: | |
本文献已被 Oxford 等数据库收录! |
|