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Caspase-2 cleaves DNA fragmentation factor (DFF45)/inhibitor of caspase-activated DNase (ICAD) |
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| Authors: | Dahal Giri Raj Karki Pratap Thapa Arjun Shahnawaz Mohammad Shin Song Yub Lee Jung Sup Cho Byungyun Park Il-Seon |
| Institution: | a Research Center for Proteineous Materials (RCPM), Department of Bio-materials Engineering, Chosun University, Gwangju 501-759, Republic of Korea b Department of Cellular and Molecular Medicine, School of Medicine, Chosun University, Gwangju 501-759, Republic of Korea c Department of Biotechnology, College of Natural Sciences, Chosun University, Gwangju 501-759, Republic of Korea d Department of Chemistry, Yonsei University, Wonju, Republic of Korea |
| Abstract: | To investigate the signal transduction pathway of caspase-2, cell permeable Tat-reverse-caspase-2 was constructed, characterized and utilized for biochemical and cellular studies. It could induce the cell death as early as 2 h, and caspase-2-specific VDVADase activity but not other caspase activities including DEVDase and IETDase. Interestingly, nuclear DNA fragmentation occurred and consistently DNA fragmentation factor (DFF45)/Inhibitor of caspase-activated DNase (ICAD) was cleaved inside the cell as well as in vitro, suggesting a role of caspase-2 in nuclear DNA fragmentation. |
| Keywords: | Caspase-2 Tat Apoptosis DNA fragmentation factor |
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