Hormonal and nutritional regulation of muscle carnitine palmitoyltransferase I gene expression in vivo |
| |
Authors: | Liu Hong Yan Zheng Guolu Zhu Hongfa Woldegiorgis Gebre |
| |
Affiliation: | Department of Environmental and Biomolecular Systems, OGI School of Science and Engineering, Oregon Health and Science University, Beaverton, OR 97006-8921, USA |
| |
Abstract: | Transgenic mice carrying the human heart muscle carnitine palmitoyltransferase I (M-CPTI) gene fused to a CAT reporter gene were generated to study the regulation of M-CPTI gene expression. When the mice were fasted for 48 h, CAT activity and mRNA levels increased by more than 2-fold in heart and skeletal muscle, but not liver or kidney. In the diabetic transgenic mice, there was a 2- to 3-fold increase in CAT activity and CAT mRNA levels in heart and skeletal muscle which upon insulin administration reverted to that observed with the control insulin sufficient transgenic mice. Feeding a high fat diet increased CAT activity and mRNA levels by 2- to 4-fold in heart and skeletal muscle of the transgenic mice compared to the control transgenic mice on regular diet. Overall, the M-CPTI promoter was found to be necessary for the tissue-specific hormonal and dietary regulation of the gene expression. |
| |
Keywords: | M-CPTI Promoter CAT Gene expression Transgenic mice Regulation Hormonal Dietary |
本文献已被 ScienceDirect PubMed 等数据库收录! |