Different effects of galactose and mannose on cell proliferation and intracellular soluble sugar levels in <Emphasis Type="Italic">Vigna angularis</Emphasis> suspension cultures |
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Authors: | Aki Kato Hiroshi Tohoyama Masanori Joho Masahiro Inouhe |
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Institution: | (1) Biology and Environmental Sciences, Graduate School of Science Engineering, Ehime University, Matsuyama Ehime, 790-8577, Japan |
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Abstract: | Plant cells utilize various sugars as carbon sources for growth, respiration and biosynthesis of cellular components. Suspension-cultured
cells of azuki bean (Vigna angularis) proliferated actively in liquid growth medium containing 1% (w/v) sucrose, glucose, fructose, arabinose or xylose, but did
not proliferate in medium containing galactose or mannose. These two latter sugars thus appeared distinct from other sugars
used as growth substrates. Galactose strongly inhibited cell growth even in the presence of sucrose but mannose did not, suggesting
a substantial difference in their effects on cell metabolism. Analysis of intracellular soluble-sugar fractions revealed that
galactose, but not mannose, caused a conspicuous decrease in the cellular level of sucrose with no apparent effects on the
levels of glucose or fructose. Such a galactose-specific decrease in sucrose levels also occurred in cells that had been cultured
together with glucose in place of sucrose, suggesting that galactose inhibits the biosynthesis, rather than uptake, of sucrose
in the cells. By contrast, mannose seemed to be metabolically inert in the presence of sucrose. From these results, we conclude
that sucrose metabolism is important for the heterotrophic growth of cells in plant suspension-cultures. |
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Keywords: | Azuki bean Cell growth Galactose inhibition Mannose metabolism Sucrose biosynthesis Suspension cultures |
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