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A nomogram method for calculating the NAD(P)+/NAD(P)H ratio in cell compartments |
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| Authors: | D A Mel'nichuk L V Skorik I M Sulima |
| Abstract: | A new method to calculate the ratios of free NAD+/NADH and NADP+/NADPH [NAD(P)+/NAD(P)H] in the cytoplasm and mitochondria of cells by means of nomographs is suggested. The method permits estimating the redox state of the tissue with allowance for the content of metabolites in the dehydrogenase systems. The method may be used widely in the biochemical and medical practice. |
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