Production of [14C]Acetylcholine and [14C]Carbon Dioxide from [U–14C]Glucose in Tissue Prisms from Aging Rat Brain |
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| Authors: | N. R. Sims K. L. Marek D. M. Bowen A. N. Davison |
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| Affiliation: | Miriam Marks Department of Neurochemistry, Institute of Neurology, London, Untied Kingdom |
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| Abstract: | Abstract: Production of [14C]acetylcholine and 14CO2 was examined by using tissue prisms from neocortex, hippocampus, and striatum from rats aged approximately 5 months, 13 months, and 27 months. [14C]Acetylcholine synthesis in the striatum showed highly significant decreases with age for measurements in the presence of both 5 m m - and 31 m m -K+, contrasting with the lack of significant change in 14CO2 production in this region. The neocortex and hippocampus showed only small changes, especially when comparison was made between 13-month and senescent animals. Measurements of the release of [14C]acetylcholine and influence of atropine on this release confirmed the relative stability with age of the cholinergic system in the neocortex. |
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| Keywords: | Acetylcholine synthesis Glucose metabolism Aging |
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