Fed-batch fermentation for production of nitrile hydratase byRhodococcus rhodochrous M33 |
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Authors: | Bu-Youn Kim Jong-Chul Kim Hyune-Hwan Lee Hyung-Hwan Hyun |
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Affiliation: | (1) R&D Center, Tong Suh Petrochemical Corp. Ltd., P.O. Box 50, Nam-Ulsan, Korea;(2) Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, 449-791 Kyunggi-do, Korea |
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Abstract: | To enhance the productivity and activity of nitrile hydratase inRhodococcus rhodochrous M33, a glucose-limited fed-batch culture was performed. In a fed-batch culture where the glucose was controlled at a limited level and cobalt was supplemented during the fermentation period, the cell mass and total activity of nitrile hydratase both increased 3.3-fold compared to that in the batch fermentation. The productivity of nitrile hydratase also increased 1.9-fold compared to that in the batch fermentation. The specific activity of nitrile hydratase in the whole cell preparation when using a fed-batch culture was 120 units/mg-DCW, which was similar to that in the batch culture. |
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Keywords: | nitrile hydratase fed-batch culture high-cell-density culture Rhodococcus rhodochrous M33 acrylamide |
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