Accurate and unambiguous tag-to-gene mapping in serial analysis of gene expression |
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| Authors: | Rodrigo Malig Cristian Varela Eduardo Agosin Francisco Melo |
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| Affiliation: | (1) Departamento de Gen?tica Molecular y Microbiolog?a, Facultad de Ciencias Biol?gicas, Pontificia Universidad Cat?lica de Chile, Alameda 340, Santiago, Chile;(2) The Australian Wine Research Institute, PO Box 197 Glen Osmond, Adelaide, SA, 5064, Australia;(3) Departamento de Ingenier?a Qu?mica y Bioprocesos, Facultad de Ingenier?a, Pontificia Universidad Cat?lica de Chile, Vicu?a Mackenna, 4860, Santiago, Chile |
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| Abstract: | Background In this study, we present a robust and reliable computational method for tag-to-gene assignment in serial analysis of gene expression (SAGE). The method relies on current genome information and annotation, incorporation of several new features, and key improvements over alternative methods, all of which are important to determine gene expression levels more accurately. The method provides a complete annotation of potential virtual SAGE tags within a genome, along with an estimation of their confidence for experimental observation that ranks tags that present multiple matches in the genome. |
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