Restoration of Escherichia coli hyd B Mutant Hydrogenase by Cultivation in Media Containing Various Metals |
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| Authors: | Koji Sode Tomoyasu Sugiyama Takehiro Yamamoto Masamitsu Tomiyama Isao Karube |
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| Affiliation: | a Research Center for Advanced Science and Technology, University of Tokyo, Meguro-ku, Tokyo, Japanb Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Midori-ku, Yokohama, Japanc Research Department, NOK Corporation, Fujisawa, Japand National Institute of Agribiological Resources, Tsukuba Science City, Ibaraki, Japan |
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| Abstract: | Hydrogenase restoration of Escherichia coli hydrogenase deficient mutant HK7, which carries a mutation at hyd B locus, was studied. Anaerobic growth of HK7 in the presence of iron chloride or vanadium chloride resulted in the restoration of hydrogen uptake activity of hydrogenase, but not hydrogen evolution activity. The growth of HK7 in the presence of nickel chloride restored total hydrogenase activity (hydrogen uptake and evolution) as Waugh and Boxer (1986) reported. Therefore, the leniency of HK7 hyd B product might permit the transportation and incorporation of iron chloride or vanadium chloride in hydrogenase, resulting in the alteration of hydrogenase activity. |
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| Keywords: | Hydrogenase Escherichia coli hyd B mutant restoration |
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