裂蹄木层孔菌子实体水提物诱导HepG2细胞凋亡的初步研究 |
| |
引用本文: | 董露璐,赵敏,安晓丽,何平平,相文华.裂蹄木层孔菌子实体水提物诱导HepG2细胞凋亡的初步研究[J].菌物学报,2009,28(3):451-455. |
| |
作者姓名: | 董露璐 赵敏 安晓丽 何平平 相文华 |
| |
作者单位: | 1. 东北林业大学生命科学学院,哈尔滨,150040 2. 中国农业科学院哈尔滨兽医研究所,哈尔滨,150001 |
| |
摘 要: | 研究裂蹄木层孔菌子实体水提物(WEPL)对人类克隆肝癌细胞系HepG2生长的作用。用裂蹄木层孔菌子实体水提物处理HepG2细胞后,噻唑蓝法(MTT法)可见浓度和时间依赖性抑制细胞增殖;电镜下观察凋亡小体的出现,流式细胞仪技术显示Annexin-Ⅴ染色呈阳性,都证明了HepG2细胞发生了凋亡。RT-PCR和Western Blot分析证实WEPL刺激Bax表达量上调、Bcl-2表达量下调进而诱导了细胞凋亡。结果表明WEPL诱发的克隆人类肝癌细胞系HepG2的细胞凋亡可能是通过上调Bax、下调Bcl-2活性来实现的。
|
关 键 词: | 大型真菌 提取物 抗肿瘤 Bax Bcl-2 |
Water extract of Phellinus linteus fruiting body induces apoptosis of HepG2 cells |
| |
Authors: | DONG Lu-Lu ZHAO Min AN Xiao-Li HE Ping-Ping XIANG Wen-Hua |
| |
Institution: | College of Life Sciences, Northeast Forestry University, Harbin 150040, China |
| |
Abstract: | The effect of water extracts of Phellinus linteus fruit bodies (WEPL) on the growth of HepG2 human colon cancer cells was investigated. Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay showed that the inhibition ratio of HepG2 cells treated with WEPL increased dose-and time-dependently. Microscopic observation proved the occurrence of apoptotic bodies. Flow cytometry detection showed the cells were positively stained by Annexin-Ⅴ. These were the hallmark features of apoptosis. The mRNA expression level and protein level of Bax were adjusted up while that of Bcl-2 were adjusted down. It seems possible that WEPL induces HepG2 cell apoptosis through down-regulating the expression of Bcl-2 gene and up-regulating the expression of Bax gene. |
| |
Keywords: | Bax Bcl-2 |
本文献已被 CNKI 万方数据 等数据库收录! |
| 点击此处可从《菌物学报》浏览原始摘要信息 |
| 点击此处可从《菌物学报》下载免费的PDF全文 |
|