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Nontransferrin-bound serum iron in thalassemia and sickle cell patients
Authors:N K Ahmed  M Hanna  W Wang
Institution:1. Department of Psychiatry and Human Behavior, Alpert Medical School, Brown University, Providence, RI, USA;2. Department of Psychology and Neuroscience, Boston College, Newton, MA, USA;3. Circadian Positioning Systems, Newport, RI, USA;4. E.P. Bradley Hospital Sleep Research Laboratory, Providence, RI, USA;1. Department of Chemical Theory of Drugs, Faculty of Pharmacy, Comenius University, Bratislava SK-83232, Slovakia;2. Department of Analytical Chemistry, Faculty of Natural Sciences, Comenius University, Bratislava SK-84215, Slovakia;3. Department of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University, Bratislava SK-83232, Slovakia;4. Faculty of Pharmacy, Comenius University, Bratislava SK-83232, Slovakia;5. Materials Research Centre, Faculty of Chemistry, University of Technology, CZ-61200 Brno, Czech Republic;1. Department of Haematology, Guy''s and St. Thomas’ NHS Foundation Trust, London, United Kingdom;2. Department of Clinical Sciences, Imperial College, London, United Kingdom;3. Department of Haematology, EBMT Data Office, Leiden, The Netherlands;4. Division of Hematology, Geneva University Hospitals, Geneva, Switzerland;5. Department of Haematology, Service d''Hématologie-Greffe, Assistance Publique Hôpitaux de Paris, Hôpital Saint-Louis, Paris, France;6. Division of Stem Cell Transplantation, University Hospital Eppendorf, Hamburg, Germany;7. Division of Hematology, University Hospital, Essen, Germany;8. Department of Haematology, Universitaetsklinikum Dresden, Dresden, Germany;9. Department of Haematology, Ospedale San Martino, Genova, Italy;10. Department of Haematology, University Hospital Leipzig, Leipzig, Germany;11. Department of Haematology, Charité Universitätsmedizin Berlin, Berlin, Germany;12. Department of Haematology, University of Freiburg, Freiburg, Germany;13. Department of Haematology, Institute of Hematology and Blood Transfusion, Prague, Czech Republic;14. Department of Haematology, HUCH Comprehensive Cancer Center, Helsinki, Finland;15. Department of Internal Medicine V, University of Heidelberg, Heidelberg, Germany;16. Department of Haematology, Universität Tübingen, Tübingen, Germany;17. Department of Haematology, King''s College Hospital, London, United Kingdom;18. Department of Haematology, CHU Nantes, Nantes, France;19. Department of Haematology, University of Münster, Münster, Germany;20. Department of Haematology, University Medical Centre, Utrecht, The Netherlands;21. Department of Stem Cell Transplantation, University Hospitals, Bristol, United Kingdom;22. Department of Haematology, Cliniques Universitaires St. Luc, Brussels, Belgium;23. Department of Haematology, Hospital Clínico Universitario de Valencia, Valencia, Spain;24. Department of Haematology, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Gliwice, Poland;25. Department of Haematology, St. James Hospital, Dublin, Ireland;26. Department of Haematology, CHU de Lille, LIRIC, INSERM U995, Universite de Lille, Lille, France
Abstract:Nontransferrin-bound iron (NTBI) was separated from transferrin bound iron (TBI) by DEAE-Sephadex-CDS filtration. TBI is eluted with Tris-NaCl buffer, NTBI that is retained on the column is eluted with citric acid. NTBI was identified in serum from thalassemia and sickle cell patients. Normal serum contained less than 6% NTBI as compared with 15-18% in patient's sera. NTBI levels were decreased significantly after 8 hr chelation with deferoxamine (DFO).
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