Effect of hypoxic stress on the immune response and the resistance to vibriosis of the shrimpPenaeus stylirostris |
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Authors: | Gilles Le Moullac Claude Soyez Denis Saulnier Dominique Ansquer Jean Christophe Avarre Peva Levy |
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Institution: | IFREMER, Aquacop, Centre Océanologique du Pacifique, BP 7004, Taravao, Tahiti, Polynésie Française |
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Abstract: | The immune response of shrimp exposed to severe hypoxia, 1 mg O2ml−1for 24 h, was measured in terms total haemocyte count (THC), differential haemocyte count (DHC), phenoloxidase activity (PO) and respiratory burst by NBT reduction. Results showed that hypoxia induced a significant decrease (P<5%) of the THC due to a significant decrease of semi-granular cells (SGC) (P<5%) and hyaline cells (HC) (P<5%). There was also a decrease in the NBT reduction. On the other hand, PO activity increased significantly (P<1%) and seemed to be related to a lower amount of plasma inhibitors regulating the prophenoloxidase system. Experimental infection with a virulentVibrio alginolyticuswas carried out by intramuscular injection of 2×104colony forming units (cfu) per shrimp. The mortality of stressed shrimp was 48% while the mortality of control shrimp was 32%. This difference was significant (P<1%). The variations of immunological parameters, except for the PO activity, seem to be consistent with thein vivoresponse against the pathogenicVibrio. Coyright 1998 Academic Press |
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Keywords: | hypoxia Penaeus stylirostris haemocyte count prophenol-oxidase respiratory burst vibriosis Vibrio alginolyticus experimental infection |
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