Rapid isolation and characterization of microsatellites from the genome of pearl oyster (Pinctada martensi Dunker) |
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Authors: | Youyi Kuang Guangxiang Tong Xuechun Yan Xiaowen Sun |
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Institution: | (1) Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Science, No. 43 Road Songfa, 150070 District Daoli, Harbin, China; |
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Abstract: | A new set of 34 microsatellite markers was isolated and characterized in pearl oyster Pincada martensi from a CA16-enriched genomic library. Of 60 microsatellite markers screened in a wild population which contained 30 individuals, 34 markers
occurred unambiguous bands, including seven monomorphic loci, 5 primer pairs (HHM844, HHM852, HHM888, HHM891, HHM899) were
duplicated. These polymorphic markers showed high genetic variability represented mainly by the number of alleles, which ranged
from 2 to 6, the expect heterozygosity which ranged from 0.1653 to 0.7622, and the polymorphic information content which ranged
from 0.1516 to 0.7279. These markers could increase the available molecular resources that can be used to analyze genetic
diversity and structure of pearl oyster. |
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Keywords: | |
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