Genetic evidence for functional specificity of the yeast GCN2 kinase |
| |
Authors: | N Tavernarakis G Thireos and N Tavernarakis |
| |
Institution: | (1) Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, PO Box 1527, 711 10 Heraklion Crete, Greece;(2) Department of Molecular Biology and Biochemistry, Center for Advanced Biotechnology and Medicine, Room 314, Rutgers, The State University of New Jersey, 679 Hoes Lane, 08855 Piscataway, New Jersey, USA |
| |
Abstract: | In yeast the GCN2 kinase mediates translational control ofGCN4 by phosphorylating the subunit of eIF-2 in response to extracellular amino acid limitation. Although phosphorylation of eIF-2 has been shown to inhibit global protein synthesis, amino acid starvation results in a specific activation effect onGCN4 mRNA translation. Under the same conditions, translation of other mRNAs appears only slightly affected. The mechanism responsible for the observed selectivity of the GCN2 kinase is not clear. Here, we present genetic evidence that suggests that locally restricted action of the GCN2 kinase facilitatesGCN4-specific translational regulation. |
| |
Keywords: | GCN4 eIF-2 DAI kinase tRNA Translational regulation |
本文献已被 SpringerLink 等数据库收录! |
|