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Two-step cleavage of hairpin RNA with 5' overhangs by human DICER |
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| Authors: | Yoshinari Ando Yoshiko Maida Ayako Morinaga Alexander M Burroughs Ryuichiro Kimura Joe Chiba Harukazu Suzuki Kenkichi Masutomi Yoshihide Hayashizaki |
| Institution: | (1) RIKEN Omics Science Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan;(2) Cancer Stem Cell Project, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan;(3) Department of Biological Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan;(4) PREST, apan Science and Technology Agency, 4-1-8 Honcho Kawaguchi, Saitama 332-0012, Japan |
| Abstract: | BackgroundDICER is an RNase III family endoribonuclease that processes precursor microRNAs (pre-miRNAs) and long double-stranded RNAs, generating microRNA (miRNA) duplexes and short interfering RNA duplexes with 20~23 nucleotides (nts) in length. The typical form of pre-miRNA processed by the Drosha protein is a hairpin RNA with 2-nt 3' overhangs. On the other hand, production of mature miRNA from an endogenous hairpin RNA with 5' overhangs has also been reported, although the mechanism for this process is unknown. |
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