| 巢式甲基化特异性PCR检测肝细胞癌患者血清中RASSF1A和CDH13基因启动子区甲基化 |
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| 引用本文： | 龚环宇,刘振国,张浩晔.巢式甲基化特异性PCR检测肝细胞癌患者血清中RASSF1A和CDH13基因启动子区甲基化[J].生命科学研究,2011,15(1):56-60. |
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| 作者姓名： | 龚环宇 刘振国 张浩晔 |
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| 作者单位： | 中南大学,湘雅三医院,感染科,中国湖南,长沙,410013 |
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| 基金项目： | 湖南省卫生厅科研基金资助项目(BD006090) |
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| 摘 要： | 为了检测肝细胞癌患者血清中RASSF1A和CDH13基因启动子的甲基化状态,收集肝细胞癌患者及健康对照者的血清标本,采用巢式甲基化特异性PCR(nMSP)法检测RASSF1A和CDH13基因启动子区甲基化状态.结果肝细胞癌患者血清样品中RASSF1A和CDH13基因启动子区甲基化率为53.12%和31.25%,68.75%的患者血清可以检测到异常甲基化,正常对照血清中未检测到RASSF1A和CDH13基因启动子区甲基化,RASSF1A和CDH13基因甲基化与患者的临床病理资料无明显相关性(P>0.05);表明nMSP法检测血清中RASSF1A和CDH13基因启动子区甲基化具有较高的敏感性,可为肝细胞癌的筛查、早期诊断和预后判断提供有价值的信息.
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| 关 键 词： | 肝细胞癌 甲基化 RASSF1A基因 CDH13基因 巢式甲基化特异性聚合酶链反应 |
Detection of Promoter Hypermethylation of RASSF1A and CDH13 Gene by Nested Methylation Specific Polymerase Chain Reaction in Hepatocellular Carcinoma Patients |
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| GONG Huan-yu,LIU Zhen-guo,ZHANG Hao-ye.Detection of Promoter Hypermethylation of RASSF1A and CDH13 Gene by Nested Methylation Specific Polymerase Chain Reaction in Hepatocellular Carcinoma Patients[J].Life Science Research,2011,15(1):56-60. |
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| Authors: | GONG Huan-yu LIU Zhen-guo ZHANG Hao-ye |
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| Institution: | GONG Huan-yu,LIU Zhen-guo,ZHANG Hao-ye(Infection Section,The Third Xiangya Hospital,Central South University,Changsha 410013,Hunan,China) |
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| Abstract: | To evaluate the methylation status of the promoter region of RASSF1A and CDH13 gene using serum samples from hepatocellular carcinoma(HCC) patients,nested methylation-specific PCR(nMSP) was used to detect RASSF1A and CDH13 promoter hypermethylation in serum DNA from 32 cases of HCC,while serum samples from 20 healthy individuals were used as controls.The positive rate of promoter hyper-methylation in RASSF1A and CDH13 gene were 53.12% and 31.25% in the serum of HCC patients.Overall,68.75% patients with HCC ... |
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| Keywords: | hepatocellular carcinoma hypermethylation RASSF1A CDH13 nested methylation-specific PCR |
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