Evaluation of three high abundance protein depletion kits for umbilical cord serum proteomics |
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| Authors: | Bin Liu Fang-hua Qiu Courtney Voss Yun Xu Ming-zhe Zhao Yan-xin Wu Jing Nie Zi-lian Wang |
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| Affiliation: | (1) Department of Obstetrics and Gynecology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, PR China;(2) Laboratory of Proteomics, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, PR China;(3) Department of Biochemistry, University of Western Ontario, London, Ontario, N6A 5C1, Canada;(4) Department of Endocrinology, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510000, PR China |
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| Abstract: | Background High abundance protein depletion is a major challenge in the study of serum/plasma proteomics. Prior to this study, most commercially available kits for depletion of highly abundant proteins had only been tested and evaluated in adult serum/plasma, while the depletion efficiency on umbilical cord serum/plasma had not been clarified. Structural differences between some adult and fetal proteins (such as albumin) make it likely that depletion approaches for adult and umbilical cord serum/plasma will be variable. Therefore, the primary purposes of the present study are to investigate the efficiencies of several commonly-used commercial kits during high abundance protein depletion from umbilical cord serum and to determine which kit yields the most effective and reproducible results for further proteomics research on umbilical cord serum. |
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