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Modified gateway system for double shRNA expression and Cre/lox based gene expression

Authors:	Nikolina Radulovich Lisa Leung Ming-Sound Tsao

Institution:	(1) Ontario Cancer Institute/Princess Margaret Hospital Site, University Health Network, 610 University Ave, M5G 2M9 Toronto, Ontario, Canada;(2) Department of Laboratory Medicine and Pathobiology, Faculty of Medicine, University of Toronto, Medical Sciences Buildings, 1 King’s College Circle, M5 S 1A8 Toronto, Ontario, Canada;(3) Department of Medical Biophysics, University Health Network, Ontario Cancer Institute/Princess Margaret Hospital Site, 610 University Ave, M5G 2M9 Toronto, Ontario, Canada

Abstract:	Background The growing need for functional studies of genes has set the stage for the development of versatile tools for genetic manipulations.

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