Protective effect of gamma-aminobutyric acid (GABA) against cytotoxicity of ethanol in isolated rat hepatocytes involves modulations in cellular polyamine levels |
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Authors: | T Norikura A Kojima-Yuasa D Opare Kennedy I Matsui-Yuasa |
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Institution: | (1) Department of Food and Human Health Sciences, Graduate School of Human Life Science, Osaka City University, Osaka, Japan |
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Abstract: | Summary. Gamma-aminobutyric acid (GABA) is considered to be a multifunctional molecule with various physiological effects throughout
the body. It is also evident that the liver contains GABA and its transporter. However, the functions of GABA in liver have
not been well documented. In this study, the cytoprotective effect of GABA against ethanol-induced hepatotoxicity was evaluated
in primary cultured rat hepatocytes. Addition of ethanol induced decrease of cell viability in a dose-dependent manner. However,
treatment with GABA resulted in a dose-dependent recovery from ethanol (150 mM)-induced cytotoxicity.
GABA reversed the ethanol-induced decrease in intracellular polyamine levels. Furthermore, the addition of polyamines also
reversed the ethanol-induced decrease of cell viability. These results suggest that GABA is protective against the cytotoxicity
of ethanol in isolated rat hepatocytes and this effect may be modulated by the maintenance of intracellular polyamine levels. |
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Keywords: | : GABA – Ethanol – Hepatocytes – Polyamines |
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