Presence of mycobacteriophage I3-like DNA sequences in the genome of its host Mycobacterium smegmatis |
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| Authors: | C Sadhu S Dutta K P Gopinathan |
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| Institution: | (1) Microbiology and Cell Biology Laboratory, Indian Institute of Science, 560012 Bangalore, India;(2) Present address: Department of Biology, University of Calgary, T2N 1N4 Calgary, Alberta, Canada;(3) Present address: Department of Cell Biology, Baylor College of Medicine, 77030 Houston, TX, USA |
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| Abstract: | The genomes of phage I3 and its host Mycobacterium smegmatis have been compared. From thermal melting studies the GC contents of DNA from mycobacteriophage I3 and its host M. smegmatis were found to be 66%. A new method, based only on the initial rates of reassociation, has been developed for calculating the DNA homology. Analysis of DNA reassociation kinetics suggested the presence of one equivalent of the phage I3 genome within the M. smegmatis genome. Southern analysis revealed the presence of almost all of the phage I3 specific sequences within the host genome. |
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| Keywords: | Mycobacterium smegmatis Mycobacteriophage I3 DNA reassociation Sequence homology |
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