Physical and functional map of the hemolytic plasmid pSU316 |
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Authors: | Isabel Andrés JoséC Rodriguez JoséM Ortiz |
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Institution: | Departamento de Bioquímica, Facultad de Medicina, Polígono de Cazoña s/n, Santander, Spain |
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Abstract: | A restriction endonuclease analysis of the hemolytic plasmid pSU316 has allowed location of the cleavage sites for the endonucleases BamHI, XbaI, KpnI, BglII, SalGI, EcoRI, and HindIII. Hybridization experiments between pSU316 and pED100 have shown that the tra region of pSU316 lies in a segment comprising part of SalGI fragments S-1 and S-3 and the entire fragment S-4. The positions of other plasmid coded functions, namely the replication functions and α-hemolysin production, have been determined in the physical map. |
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Keywords: | To whom correspondence should be sent |
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