UDN glycoprotein inhibits activator protein-1 and matrix metalloproteinase-9 via blocking of oxygen radicals in HT-29 cells |
| |
Authors: | Sei-Jung Lee Kye-Taek Lim |
| |
Institution: | (1) Molecular Biochemistry Laboratory, Biotechnology Research Institute and Center for the Control of Animal Hazards Using Biotechnology (BK 21), Chonnam National University, 300 Yongbong-Dong, Kwang-ju, 500-757, South Korea;(2) Molecular Biochemistry Laboratory, Institute of Biotechnology, Chonnam National University, 300 Yongbong-Dong, Kwang-ju, 500-757, South Korea |
| |
Abstract: | The present study was performed to investigate the anti-inflammatory potential of a 116-kDa glycoprotein isolated from Ulmus davidiana Nakai (UDN glycoprotein) in lipopolysaccaride (LPS)-treated cancerous human colon epithelial cells (HT-29 cells). UDN glycoprotein
inhibited the production of intracellular superoxide anion (O2·−), hydrogen peroxides (H2O2), and nitric oxide (NO), whereas normalized the activity of anti-oxidant enzymes superoxide dismutase (SOD), catalase (CAT),
and glutathione peroxidase (GPX)], accompanying the inhibition of manganese-superoxide dismutases (Mn-SOD) activity in LPS-treated
HT-29 cells. In addition, UDN glycoprotein blocked the DNA binding activity of activator protein-1 (AP-1) through suppression
of c-Jun and c-Fos activities, respectively. We also evaluated the anti-inflammatory potential of UDN glycoprotein based on
the activity of the pro-inflammatory signal mediators inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and
matrix metalloproteinases-9 (MMP-9)]. The results showed that UDN glycoprotein (200 μg/ml) has an inhibitory effect on the
activation of iNOS, COX-2, and MMP-9 proteins in the LPS-treated HT-29 cells. From these results, we suggest that UDN glycoprotein
is one of the potential anti-inflammatory agents that blocks LPS-mediated inflammatory signal pathway in HT-29 cells. Here,
we speculate that UDN glycoprotein could be used as an antioxidative agent for inflammatory gastrointestinal cancers. |
| |
Keywords: | UDN glycoprotein Manganese-superoxide dismutases Activator protein-1 Matrix metalloproteinases-9 HT-29 cells |
本文献已被 PubMed SpringerLink 等数据库收录! |
|