Abscisic acid binding to subcellular fractions from leaves of Vicia faba |
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Authors: | Hocking T J Clapham Jennifer Cattell K J |
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Institution: | (1) Department of Biological Sciences, The Polytechnic, Wulfruna Street, WV1 ILY Wolverhampton, U.K. |
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Abstract: | A centrifugation binding assay has been used to demonstrate the binding of 3H] (±) abscisic acid to membrane-rich fractions prepared from leaves of Vicia faba L. Kinetic analysis of this binding shows evidence of saturation of binding sites with increasing concentration of ligand. Scatchard analysis of these data yields a biphasic plot possibly indicating the presence of two types of binding sites. The dissocation constant for the high affinity site has been calculated to be 3.5×10-8 mol 1-1. |
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Keywords: | Abscisic acid Binding assay Hormone binding Plasma membranes Vicia |
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