乳酸菌源有机硒对肝损伤小鼠脾脏脂质过氧化和NK细胞活性的保护效应研究

本文通过研究乳酸菌源有机硒干预CCl_4致肝损伤小鼠脾脏NK细胞活性和脂质过氧化反应的变化,探讨该有机硒在抗损伤保护过程中的效应及其机制。分别选用60只健康成年小鼠,雌雄对半,随机分成对照组(C组),有机硒组(Se组),CCl_4组、CCl_4-有机硒保护组(CCl_4-Se组),每组15只。通过腹腔注射CCl_4诱发肝损伤后,分别在第2、4周检测脾脏NK细胞活性及其组织匀浆GSH-Px、CAT、SOD活性和MDA含量变化、结果显示,在整个实验期内,C组、Se组和CCl_4-Se组脾组织匀浆GSH—Px、CAT和SOD活性均高于或明显高于CCl_4组,Se和CCl_4-Se组与C组比较除SOD活性在第4周有明显升高外均差异不显著;CCl_4组小鼠脾脏MDA含量均显著高于C组、Se组和CCl_4-Se组,而CCl_4-Se组与C组接近,Se组较CCl_4-Se组和C组低;Se组NK细胞活性最高,第4周明显高于C组,CCl_4组最低且低于或明显低于CCl_4-Se、Se和C组,CCl_4-Se组与C组无明显差异。结果提示,乳酸菌源有机硒能够提高正常机体抗氧化能力,在干预肝损伤过程中,可以通过改善和提高脾组织抗氧化酶活性及NK细胞活性发挥积极有效的作用。

PROTECTIVE EFFECT OF ORGNOSELENIUM FROM Se-ENRICHED LACTOBACILLUS ON LIPID PEROXIDATION REACTION AND NK CELL ACTIVITY IN SPLEEN OF LIVER INJURY MICE

Changes of lipid peroxidation reaction and NK cell activity in spleen of CCl_4-in- duced liver injury mice with adding orgnoselenium from Se-enriched lactobacillus were studied to discuss the protective effect of orgnoselenium and its mechanism. Sixty healthy mice (female: male=1:1) were chosen and divided randomly into four groups: control group(group C), orgnoselenium group (group Se), CCl_4-injection group (group CCl_4) and CCl_4-injection plus orgnoselenium group (group CCl_4-Se). The liver injury was induced by abdominal injection of CCl_4 (0.07ml/100g body weight) on every other day over four weeks. The spleens were collected at the 2nd and 4th week, and spleen NK cell activity,spleen homogenate GSH-Px, SOD, CAT activities and MDA concen- tration were determined. The results showed that during the entire experimental period, spleen homogenate GSH-Px, SOD and CAT activities in groups C, Se and CCl_4-Se were higher or sig- nificantly higher than that in group CCl_4, and three antioxidant enzyme activities in groups Se and CCl_4-Se had no apparent differences from that in group C except that there were significant in- creases of SOD activity at the 4th week. Spleen homogenate MDA content of group CCl_4 in- creased markedly compared with that of groups C, Se and CCl_4-Se, MDA level of group CCl_4-Se was close to that of group C, and that of group Se was lower. During the entire experimental peri- od, NK cell activity of group Se was the highest and significantly higher than that of group C at the 4th week, a lowest value was observed in group CCl_4, which was lower or markedly lower than that of groups C, Se and CCl_4-Se, there were no significant differences between group CCl_4- Se and group C. It is suggested that orgnoselenium from Se-enriched lactobacillus can enhance antioxidation ability in normal mice and play an effective role by means of improving and enhanc- ing the spleen antioxidation enzymes and NK cell activities in the process of intervening liver in- jury.