Embryogenic cultures of the leguminous tree Albizia julibrissin and recovery of plants |
| |
Authors: | Austin Burns J Wetzstein Hazel Y |
| |
Institution: | (1) Horticulture Department, University of Georgia, 1111 Plant Sciences Building, Athens, GA 30602-7273, USA;(2) Present address: Monsanto Co., 700 Chesterfield Pkwy. North, St. Louis, MO, 63198, USA) |
| |
Abstract: | A somatic embryogenic system was developed and plants regenerated in mimosa (Albizia julibrissin Durazz). Development of somatic
embryos in the species has not previously been reported. Immature seeds, embryo cotyledons and embryo axes (cotyledons removed)
at defined developmental stages were placed on induction media with different concentrations of 2,4-D. Two distinct embryogenic
responses occurred: either proembryo masses or cotyledonary-stage embryos. Twenty five percent of all embryo axes cultured
on basal medium produced cotyledonary somatic embryos. Six percent of in ovulo immature seed explants generated proembryo
masses. These masses proliferated in liquid culture in the dark. Proembryos developed further when transferred to a growth-regulator-free
semisolid medium in the light. Somatic embryos derived from either proembryo suspensions or cotyledonary embryo cultures on
semisolid medium germinated to form plants that continued to grow vigorously following transfer to soil.
This revised version was published online in June 2006 with corrections to the Cover Date. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|