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| 以精子为载体的体外转基因猪胚胎研究 | |
| 引用本文： | 徐飞良,肖兵南,李红,燕海峰,邓缘,张健.以精子为载体的体外转基因猪胚胎研究[J].激光生物学报,2006,15(2):175-178. |
| 作者姓名： | 徐飞良 肖兵南 李红 燕海峰 邓缘 张健 |
| 作者单位： | 1. 湖南农业大学动物科技学院,湖南,长沙,410128;湖南省畜牧兽医研究所,湖南,长沙,410131 2. 湖南省畜牧兽医研究所,湖南,长沙,410131 3. 湖南师范大学生命科学学院基因敲除实验室,湖南,长沙,410081 |
| 基金项目： | 湖南省教育厅专项基金，湖南省财政厅专项基金，湖南省科技厅专项基金，湖南省发改委专项基金 |
| 摘 要： | 去精清精子与外源DNA共孵育,使其携带外源DNA,再与成熟卵母细胞进行体外受精,生产转基因猪胚胎,为通过胚胎移植生产转基因猪奠定基础。结果:通过PCR从不同时期胚胎中检测出携带外源DNA的阳性胚,说明精子与外源DNA共孵育能使精子携带外源DNA,并通过体外受精技术使外源DNA进入早期胚胎。 |
| 关 键 词： | 精子载体 体外受精 转基因猪胚胎 |
| 文章编号： | 1007-7146（2006）02-0175-04 |
| 收稿时间： | 2005-12-10 |
| 修稿时间： | 2005年12月10 |
Generation of Transgenic Pig Embryos by Sperm Mediated Gene Transfer |
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| XU Fei-liang,XIAO Bing-nan,LI Hong,YAN Hai-feng,DENG Yuan,ZHANG Jian.Generation of Transgenic Pig Embryos by Sperm Mediated Gene Transfer[J].ACTA Laser Biology Sinica,2006,15(2):175-178. | |
| Authors: | XU Fei-liang XIAO Bing-nan LI Hong YAN Hai-feng DENG Yuan ZHANG Jian |
| Institution: | 1. College of Animal Science in Hunan Agriculture University, Changsha 410128, China; 2. Hunan Institute of Animal and Veterinary Science, Changsha 410128, China; 3. Gene Knockout Lab, College of Life Science, Hunan Normal University, Changsha 410081 . China |
| Abstract: | Sperms which were removed of seminal fluid,were incubated with exogenous DNA,thus they can intake exogenous DNA and fertilize with matured oocytes in vitro.The purpose is to develop a way to produce early transgenic pig embryos at lower costs.It indicated that sperm incubated with exogenous DNA could intake the DNA and could be detected in embryos at different stages. |
| Keywords: | sperm mediated gene transfer in vitro fertilization transgenic pig embryos |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |