|
|||||
|
|
Improved procedure of purification of inorganic pyrophosphatase from Bacillus stearothermophilus. |
|
| Authors: | E Schreier M B El Samei |
| Abstract: | An improved method for isolation of inorganic pyrophosphatase (EC 3.6.1.1) from Bacillus stearothermophilus is described. The enzyme was purified to more than 90% after two chromatographic steps. A molecular weight of 140,000 daltons was estimated by density gradient centrifugation. The isoelectric point was found to be 4.0. |
| Keywords: | |