Characterization and distribution of multiple antigens on N-linked oligosaccharides of Dictyostelium discoideum proteins |
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| Authors: | H S Judelson H H Freeze R L Dimond |
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| Institution: | 1. Department of Bacteriology, University of Wisconsin, Madison, Wisconsin, 53706, U.S.A.;2. Cancer Center, University of California, San Diego, La Jolla, California 92093 U.S.A.;1. Department of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta, GA;2. Department of Pediatrics, University of Florida College of Medicine, Gainesville, FL;3. Department of Obstetrics and Gynecology, University of Florida College of Medicine, Gainesville, FL;4. Department of Epidemiology, University of Florida College of Public Health and Health Professions and College of Medicine, Gainesville, FL;1. G.I.K. Institute of Engineering Sciences and Technology, Topi 23460, Swabi, Khyber Pakhtunkhwa, Pakistan;2. Machine Learning and Data Science Lab (MDS), G.I.K. Institute, Pakistan;3. Electrical and Computer Engineering Department, Effat University, 22332 Jeddah, Saudi Arabia;1. Joint International Research Laboratory of Metabolic and Developmental Sciences, State Key Laboratory of Hybrid Rice, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 20040, China;2. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, China;3. College of Life Science, Ningxia University, Yinchuan 750021, China;4. Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in Western China, Ningxia University, Yinchuan 750021, China;5. Crop Biotech Institute and Graduate School of Biotechnology, Kyung Hee University, Yongin 17104, Korea;6. Department of Plant & Environmental Sciences, University of Copenhagen, 1871 Frederiksberg C, Denmark;7. Copenhagen Plant Science Center, University of Copenhagen, 1871 Frederiksberg C, Denmark;8. School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Urrbrae, SA 5064, Australia;1. Department of Horticultural Sciences, Seoul Women’s University, Nowon-gu, Seoul 01797, Republic of Korea;2. Virology Unit, Horticultural and Herbal Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju 55365, Republic of Korea;1. Department of Cell Biology, Faculty of Medicine & Dentistry, University of Alberta, Edmonton, Canada;2. Department of Medical Microbiology & Immunology, Faculty of Medicine & Dentistry, University of Alberta, Edmonton, Canada;3. Department of Biochemistry, Microbiology and Immunology, University of Saskatchewan, Saskatoon, Canada;4. Vaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, Saskatoon, Canada;5. Department of Veterinary Microbiology, University of Saskatchewan, Saskatoon, Canada;6. Department of Biochemistry, McGill University, Montreal, Canada;7. Li Ka Shing Institute of Virology, University of Alberta, Edmonton, Canada;1. Department of Ophthalmology, The Affiliated Hospital of Southwest Medical University, Luzhou, 646000, PR China;2. Department of Rheumatology and Immunology, The Affiliated Hospital of Southwest Medical University, PR China;3. The Aier Eye Hospital of Kunming, PR China;4. Department of Ophthalmology, The People''s Hospital of Leshan, PR China |
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| Abstract: | Monoclonal antibodies were prepared against a mixture of purified lysosomal enzymes from Dictyostelium discoideum. Three classes of antibodies were found which recognized distinct antigenic determinants on N-linked oligosaccharides of multiple proteins. The structure of the determinants was studied by competition assays using monosaccharides and oligosaccharide/glycopeptide fractions prepared from one Dictyostelium lysosomal enzyme or other sources. The results of these studies suggest that one class of antibody recognizes an epitope containing residues of Man-6-SO4, another recognizes a domain containing a modified GlcNAc, and the third class recognizes an undefined determinant that involves the oligosaccharide. The three determinants are found on multiple overlapping, but nonidentical sets of glycoproteins. The ability to produce monoclonal antibodies against unusual N-linked oligosaccharides offers a powerful tool which can be used to investigate the occurrence, structure, biosynthesis, and the biological roles of these highly immunogenic saccharides. |
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