In vitro propagation of mature trees of Alnus incana (L.) Moench. |
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Authors: | P. Périnet G. Vallée F. M. Tremblay |
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Affiliation: | (1) Les Laboratoires Rhizotec Inc., C.P. 797, G6Z 2L9 St-Jean-Chrysostome, Québec, Canada;(2) Present address: Canadian Forestry Service, Petawawa National Forestry Institute, K0J 1J0 Chalk River, Ontario, Canada;(3) Direction de la Recherche et du Développement, Ministère de l'Energie et des Ressources Québec, 2700 Einstein, G1P 3W8 Ste-Foy, Québec, Canada |
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Abstract: | Mature trees of European grey alder (Alnus incana) were micropropagated on a modified MS medium containing 2.5 M BA, 6.2 mM (500 mg l-1) NH4NO3 and 1.5% glucose. Prior to in vitro culture, mature scions were multiplied through grafting and cutting techniques. Shoot tips from cuttings were established in vitro. After six months of culture, shoots were rooted either in vitro or in vivo and plantlets were transferred to greenhouse conditions. |
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Keywords: | Alnus incana alder micropropagation mature trees |
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