Distribution of acetyl esterase in wood-rotting fungi |
| |
Authors: | Sho-ichi Tsujiyama Naoko Nakano |
| |
Institution: | (1) Faculty of Agriculture, Kyoto Prefectural University, Shimogamo-nakaragi-cho, Sakyo-ku, 606 Kyoto, Japan;(2) Present address: Arborex Co. Ltd., 587 Osaka, Japan |
| |
Abstract: | The distribution of acetyl esterase was studied in 30 strains of wood-rotting fungi. A screening test on agar plates using
glucose β-d-pentaacetate as a substrate indicated that all tested fungi produced acetyl esterase to form a clear zone on the culture.
All fungi also showed positive responses in an agar test using carboxymethyl cellulose acetate. Enzyme assay showed that extracellular
acetylxylan esterase activity was present in the filtrates of wood-meal culture of all these fungi. The ratio of fungal acetylxylan
esterase activity to 4-nitrophenyl acetyl esterase activity were higher than that of porcine liver esterase, indicating that
fungal esterases have high affinity for acetylated carbohydrates. Acetyl esterase is suggested to be distributed widely in
wood-rotting fungi for degradation of native acetylated hemicelluloses. |
| |
Keywords: | acetyl esterase wood biodegradation wood-rotting fungi |
本文献已被 SpringerLink 等数据库收录! |