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Mixis strategies and resting eeg production of rotifers living in temporally-varying habitats
Authors:Serra  Manuel  Carmona  Maria José
Institution:(1) Associatition ‘Leiden-Camargue’, Station Biologique de la Tour du Valat, Le Sambuc, 13200 Arles, France
Abstract:The organic phosphate pool of some Camargue sediments (South of France) was studied, after removal of inorganic phosphate, with Ca-NTA/dithionite (Fe bound phosphate) and Na-EDTA (Ca bound phosphate). The organic phosphate was divided into an acid soluble organic phosphate fraction (ASOP) and a residual organic phosphate fraction (ROP). The extraction of organic matter with 2.0 M NaOH (90 °C) from ROP yielded considerable quantities of Org-P. In this extract the presence of phytate (inositol hexa phosphate) could be demonstrated using phytase to hydrolyse the phytate. Phytate was shown to account for a considerable part of organic phosphate in sediments of freshwater marsh sediments as well as in the sediment of the brackish/salt water lake ‘Etang de Vaccares’. In laboratory experiments phytate was found to precipitate with all poly-valent cations tested. Furthermore, phytate was found to be strongly adsorbed onto Fe(OOH), which may explain its accumulation and its stability in sediments. Considerable quantities of ASOP were found; the chemical stucture of this pool remains unknown.
Keywords:phytate  organic phosphate  sediment  P-fractionation  Camargue
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