Acetylcholine,GABA and glutamate induce ionic currents in cultured antennal lobe neurons of the honeybee, <Emphasis Type="Italic">Apis mellifera</Emphasis> |
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Authors: | Guillaume?Stephane?Barbara Christina?Zube Jürgen?Rybak Monique?Gauthier Email author" target="_blank">Bernd?GrünewaldEmail author |
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Institution: | 1.Institut für Biologie, AG Neurobiologie,Freie Universit?t Berlin,Berlin,Germany;2.Centre de Recherches sur la Cognition Animale-CNRS,Universite Paul Sabatier,Toulouse,France;3.Department of Behavioural Physiology and Sociobiology (Zoologie II),Universit?t Würzburg Biozentrum,Würzburg,Germany |
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Abstract: | The honeybee, Apis mellifera, is a valuable model system for the study of olfactory coding and its learning and memory capabilities. In order to understand
the synaptic organisation of olfactory information processing, the transmitter receptors of the antennal lobe need to be characterized.
Using whole-cell patch-clamp recordings, we analysed the ligand-gated ionic currents of antennal lobe neurons in primary cell
culture. Pressure applications of acetylcholine (ACh), γ-amino butyric acid (GABA) or glutamate induced rapidly activating
ionic currents. The ACh-induced current flows through a cation-selective ionotropic receptor with a nicotinic profile. The
ACh-induced current is partially blocked by α-bungarotoxin. Epibatidine and imidacloprid are partial agonists. Our data indicate
the existence of an ionotropic GABA receptor which is permeable to chloride ions and sensitive to picrotoxin (PTX) and the
insecticide fipronil. We also identified the existence of a chloride current activated by pressure applications of glutamate.
The glutamate-induced current is sensitive to PTX. Thus, within the honeybee antennal lobe, an excitatory cholinergic transmitter
system and two inhibitory networks that use GABA or glutamate as their neurotransmitter were identified. |
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Keywords: | Insect Neurotransmitter Acetylcholine receptor Patch clamp Glutamate receptor |
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