外源~1O_2 和·OH及其清除剂对烟草愈伤组织交替途径发生与运行的影响(英文)

外源1 O2 和·OH处理继代培养 1 4d的烟草愈伤组织 2 4h后 ,愈伤组织内的交替途径的实际运行显著上升 ,但对交替途径容量影响不大 ;而经·OH处理后的愈伤组织交替途径容量和实际运行均明显地受到抑制 ,但交替途径的实际运行对·OH更加敏感。活性氧产生系统中加入相关清除剂进行实验 ,得到与此相对应的结果 :1 O2 的清除剂His处理能明显地降低或抑制1 O2 所增加的交替途径实际运行量 ,但对交替途径容量则几乎均无影响 ,而用·OH的清除剂DMSO和MAN分别处理愈伤组织后 ,DMSO和MAN均能解除·OH对交替途径容量和实际运行的抑制。这些结果表明 ,1 O2 诱导烟草愈伤组织交替途径的实际运行 ,但对其容量的作用不大 ,而·OH则明显地抑制交替途径容量和实际运行。推测1 O2 和·OH对抗氰途径的影响可能是通过对AOX活性的调节

Effects of Exogenous ~1O_2 and Their Scavengers on the Capacity and Operation of the Alternative Respiratory Pathway in Tobacco Callus

In tobacco (Nicotiana rustica L.. Gansu Yellow Flower) callus,addition of exogenous 1O2 in- duced a pronounced increase in the operation activity of alternative respiratory pathway without affecting its capacity. The operation activity of alternative pathway activated by1O2can be abolished by its scavenger (His). Exogenous -OH diminished the operation ac- tivity and capacity of alternative pathway, which could be recovered by the addition of ·OH scaven- gers, such as DMSO or MAN.? These observations suggest that1O2is involved in some way in the activa- ting the alternative pathway, but not in the induction of the capacity of alternative pathway; but ·OH can suppress both the actual operation activity and capaci- ty of alternative pathway remarkably.e deduced that 1O2 and ·OH might affect the alternative pathway by regulating the activity of the alternative oxidase.